Introduction

This study aimed to compare the performance of the Kyratec SC200 SuperCycler and Biometra Tpersonal PCR machines in a controlled environment. Four experiments were conducted in total, including two utilising replicates of the M13 MCS region of pTZ57R (first placed at random in the SuperCycler, then in a row in the Supercycler), and two utilising replicates of plant chloroplast DNA (1.5kb and 360bp respectively).

Experiment 1:

15 replicates of M13 MCS region of pTZ57R were amplified with an annealing temperature of 52°C. 12 replicates were placed at random locations across the 96 well block of the Kyratec SuperCycler. 3 replicates were placed in a Biometra Tpersonal PCR to serve as control.

Results:

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Experiment 2:

15 replicates of M13 MCS region of pTZ57R were amplified with an annealing temperature of 52°C. 12 replicates were placed in a row across the 96 well block Kyratec SuperCycler. 3 replicates were placed in a Biometra Tpersonal PCR to serve as control.

Result:

Legend:

Experiment 3:

15 replicates of plant chloroplast DNA (1.5kb) were amplified with an annealing temperature of 52°C. 12 replicates were placed in a row of across the 96 well block of the SuperCycler. 3 replicates were placed in a Biometra Tpersonal PCR machine to serve as a control.

Result:

Legend:

Experiment 4:

15 replicates of plant chloroplast DNA (360bp) were amplified with an annealing temperature of 52°C. 12 replicates were placed in a row of across the 96 well block of the Kyratec SuperCycler. 3 replicates were placed in a Biometra Tpersonal PCR machine to serve as a control.

Result:

 

Legend:

Conclusion:

The experiments above prove that the Kyratec SuperCycler yields results which are comparable in quality to those of the Biometra Tpersonal PCR machine.